TitleStructural Studies of Planctomycete Gemmata obscuriglobus Support Cell Compartmentalisation in a Bacterium
Publication TypeJournal Article
Year of Publication2014
AuthorsSagulenko E., Morgan GP., Webb RI., Yee B., Lee K-C., Fuerst JA.
Date PublishedMar 14
Type of ArticleArticle
ISBN Number1932-6203
Accession NumberBIOSIS:PREV201400322784
Keywords02502, Cytology - General, 10060, Biochemistry studies - General, 30500,, 31000, Physiology and biochemistry, Bacteria, Eubacteria, Microorganisms, Biochemistry and Molecular Biophysics, Budding and Appendaged Bacteria, Eubacteria, Bacteria, Microorganisms, Cell Biology, cell division, cell organization, evolutionary consequence, FtsK protein, immunogold localization, laboratory techniques, laboratory techniques, immunologic, mathematical and computer, Morphology and cytology of bacteria, Nonprosthecate Bacteria [08320], nuclear body/riboplasm, of bacteria, planctomycete], techniques/electron tomography, techniques/three-dimensional model, [Gemmata obscuriglobus
AbstractMembers of phylum Planctomycetes have been proposed to possess atypical cell organisation for the Bacteria, having a structure of sectioned cells consistent with internal compartments surrounded by membranes. Here via electron tomography we confirm the presence of compartments in the planctomycete Gemmata obscuriglobus cells. Resulting 3-D models for the most prominent structures, nuclear body and riboplasm, demonstrate their entirely membrane - enclosed nature. Immunogold localization of the FtsK protein also supports the internal organisation of G. obscuriglobus cells and their unique mechanism of cell division. We discuss how these new data expand our knowledge on bacterial cell biology and suggest evolutionary consequences of the findings.